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In vivo induction of endogenous retroviruses in BALB/c mouse hepatocytes by successive treatments with carbon tetrachloride and bromodeoxyuridine
Author(s) -
Ayukawa Hannah M.,
Saltzburg Donna,
Mukherjee Barid B.
Publication year - 1979
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910240419
Subject(s) - bromodeoxyuridine , carbon tetrachloride , endogeny , biology , microbiology and biotechnology , in vivo , endogenous retrovirus , nucleic acid , balb/c , virology , cell growth , biochemistry , immunology , chemistry , gene , immune system , genetics , organic chemistry , genome
The effects of bromodeoxyuridine (BrdUrd) incorporation into hepatocytes of carbon tetrachloride (CCl 4 )‐induced regenerating liver of BALB/c mice on the induction of endogenous retroviruses was examined. From the nucleic acid hybridization studies, the maximum levels of hybridization were obtained for both N‐ and X‐tropic BALB/c endogenous retrovirus specific [ 3 H]‐cDNAs with liver RNA from animals receiving BrdUrd at 40 and 44 h post‐CCl 4 treatment, and killed on the fourth day following BrdUrd injection. Media from NIH‐3T3 (Swiss mouse) and mink cell cultures, infected with liver homogenates from animals treated as above, gave significant levels of reverse transcriptase activity. The observations made in the present study show that BrdUrd incorporation into cell DNA can cause induction of both N‐ and X‐tropic endogenous retroviruses in BALB/c mouse hepatocytes in vivo , and such induction is probably a transient event.