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Malignant behaviour of three adenovirus‐2‐transformed brain cell lines and their methyl cellulose‐selected sub‐clones
Author(s) -
Gallimore Phillip H.,
McDougall James K.,
Chen Lan Bo
Publication year - 1979
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910240416
Subject(s) - fibronectin , cell culture , biology , microbiology and biotechnology , in vitro , cell , embryo , cell growth , ratón , metastasis , adenoviridae , fibronectins , pathology , immunology , genetics , medicine , cancer , gene , recombinant dna
Three adenovirus‐2‐transformed rat embryo brain cell lines and their methylcellulose‐selected subclones were examined for fibronectin expression, anchorage‐independent growth, saturation density, T antigen expression and morphology. Tumorigenicity studies were carried out on newborn and ATS immunosuppressed syngeneic rats and congenitally athymic nude mice. With one exception the methyl‐cellulose sub‐clones contained significantly fewer fibronectin‐positive cells than the parent lines; a number of sub‐clones contained no fibronectin‐positive cells. Methylcellulose selection did not always alter cell morphology, saturation density or anchorage‐independent growth as compared with parent lines. However, the methylcellulose sub‐clones were considerably more malignant than the parent cell lines as measured by invasion and metastasis in nude mice. No in vitro characteristic correlated with malignant behaviour.