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Biochemical studies during aflatoxin B 1 ‐induced liver damage in rats fed different levels of dietary protein
Author(s) -
Mirmomeni M. H.,
Suzangar M.,
Wise A.,
Messripour M.,
Emami H.
Publication year - 1979
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910240415
Subject(s) - aflatoxin , alkaline phosphatase , medicine , glutamate dehydrogenase , endocrinology , transaminase , carcinogen , mycotoxin , biology , excretion , chemistry , enzyme , food science , biochemistry , glutamate receptor , receptor
Aflatoxin B 1 has been fed for 6 months to young rats in low or high protein diets. In agreement with previous research, it was found that the high protein diet was associated with hyperplastic activity in the liver, of a type similar to that usually found in rats developing aflatoxin B 1 ‐induced hepatoma. During the same period, negligible precancerous‐like changes were seen in the rats fed the low protein diet with aflatoxin B 1 . This model has been used to test the sensitivity of various liver function tests to the dietary‐induced difference in liver reaction to aflatoxin B 1 feeding. The serum enzymes lactic dehydrogenase and alkaline phosphatase were considerably elevated in rats with precancerous‐like lesions, but the former was the most sensitive enzyme. Serum glutamate‐oxalate transaminase and serum glutamate‐pyruvate transaminase were both raised in the rats with precancerous‐like lesions, but much less so than lactic dehydrogenase and alkaline phosphatase. Urinary aflatoxin metabolites were also measured. Aflatoxin M 1 and aflatoxin P 1 were both found after feeding aflatoxin B 1 . During the feeding period, aflatoxin P 1 excretion steadily increased, while aflatoxin M 1 increased between the second and fourth months and then fell. In the first 4 months, rats fed a low protein diet tended to produce a lower ratio of aflatoxin M 1 to aflatoxin P 1 compared to the rats fed a high protein diet. At 6 months, the ratio decreased markedly, especially in the rats with the precancerous‐like lesions. It was concluded that lactic dehydrogenase, alkaline phosphatase, and the ratio of urinary excretion of aflatoxin M 1 to aflatoxin P 1 could be useful tests for inclusion in a diagnostic procedure for aflatoxin B 1 ‐induced precancerous liver changes that might be expected in human studies.