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Increased monocyte‐mediated cytostasis of lymphoid cell lines in breast and lung cancer patients
Author(s) -
Jerrells Thomas R.,
Dean Jack H.,
Richardson Gary,
Can Grace B.,
Herberman Ronald B.
Publication year - 1979
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910230606
Subject(s) - cytostasis , cancer cell , lung cancer , immunology , cancer research , thymidine , biology , cell culture , peripheral blood mononuclear cell , cell growth , growth inhibition , monocyte , cancer , medicine , cytotoxicity , in vitro , biochemistry , genetics
Peripheral blood mononuclear cells of most normal adults and patients with breast or lung cancer were found to inhibit [ 3 H]thymidine uptake by lymphoid cell lines in a growth inhibition assay. At effector target cell ratios between 5:1 and 20:1, lung cancer patients and breast cancer patients, when compared to normal individuals, demonstrated significantly greater inhibition of [ 3 H] thymidine uptake by a human lymphoid cell line (F‐265). The effector cells were adherent and were probably monocytes. Sephadex G‐10 column passage or adherence to plastic removed most growth‐inhibitory activity. Adherent cells recovered from plastic flasks (88–94% monocytes) were strongly growth‐inhibitory. Lung cancer patients receiving BCG immunotherapy were found to have an apparently increased activity compared to patients not treated with BCG. The possible mediation of the growth inhibition by release of prostaglandins was suggested by the reduced cytostatic effects in the presence of indomethacin. Growth‐inhibitory activity was not species‐restricted, since human effector cells and also effector cells from tumor‐bearing mice were reactive against the human target cell and against a murine lymphoma line (RBL‐5). Natural killer (NK) cells did not appear to contribute appreciably to the observed cytostasis, since the levels of their activities did not correlate, and human NK cells are non‐adherent and have little reactivity against F‐265 or RBL‐5. The inhibition of [ 3 H]thymidine uptake by target cells was demonstrated to be a good reflection of actual inhibition of proliferation, since incubation of adherent cells from cancer patients with F‐265 resulted in similar degrees of reduction in the number of target cells and in [ 3 H]thymidine uptake.