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Assessment of the use of the Salmonella mutagenesis assay to determine the influence of antioxidants on carcinogen‐induced mutagenesis
Author(s) -
Rosin Miriam P.,
Stich H. F.
Publication year - 1979
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910230521
Subject(s) - butylated hydroxyanisole , cysteamine , sodium bisulfite , carcinogen , chemistry , propyl gallate , antioxidant , biochemistry , mutagenesis , butylated hydroxytoluene , disulfiram , mutagen , antimutagen , organic chemistry , mutant , gene
The Salmonella mutagenesis assay was used to study the influence of antioxidants on the mutagenic activities of carcinogens. The assay allowed us to determine which antioxidant would affect a carcinogen's in vitro mutagenic activity as well as to estimate the relative effectiveness of each antioxidant as a carcinogen inhibitor. Antioxidants studied included cysteamine, disulfiram, butylated hydroxyanisole, propyl gal late, sodium selenite, sodium bisulfite, α‐tocopherol succinate and sodium ascorbate. The mutagenic activity of N ‐methyl‐ N '‐nitro‐ N ‐nitrosoguanidine was most effectively inhibited by cysteamine and sodium bisulfite with selenite, propyl gallate and sodium ascorbate being only slightly less effective. Propyl gal late, selenite and cysteamine had a similar ability to inhibit N ‐acetoxy‐2‐acetylaminofluorene‐induced mutagenesis, whereas the addition of sodium bisulfite, sodium ascorbate and disulfiram had no detectable inhibitory effect on this carcinogen. Butylated hydroxyanisole and α‐tocopherol succinate did not affect the mutagenic activity of either carcinogen. It was also observed that dimethyl sulfoxide, when used as a solvent, could significantly affect the measurement of an antioxidant's efficiency as an inhibitor of carcinogen‐induced mutagenesis. The results obtained indicate the necessity of studying an antioxidant's effect on numerous carcinogens prior to drawing any conclusions as to the potential use of the antioxidant as a carcinogen inhibitor.