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Inverse correlation between cell‐surface adhesiveness and malignancy in mouse fibroblastoid cell lines
Author(s) -
Bubeník Jan,
Perlmann Peter,
Fenyö Eva Maria,
Jandlová Táň,
Šuhajová Eva,
Malkovský Miroslav
Publication year - 1979
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910230318
Subject(s) - cell culture , clone (java method) , cell , malignancy , microbiology and biotechnology , chemistry , biology , genetics , biochemistry , dna
The correlation between tumorigenicity and cell‐surface adhesiveness was investigated in eight mouse fibroblastoid cell lines. Four of the lines (Mc 11‐Mc 15) were derived from mouse MC‐induced fibrosarcomas, two were derivatives of L cells (A9, A9HT), and two (clone 3 and clone 7 H7F4) were obtained by fusion of A9HT cells with normal diploid mouse lymphocytes. Quantitative comparison of cellular adhesive properties by the Latex particle adherence assay indicated that higher malignancy of some lines (Mc 11, 13, 14, A9HT cl 7 H7F4) was regularly associated with lower cell‐surface adhesiveness and, conversely, lower malignancy of the other lines (Mc 15, A9, cl 3) with high cell‐surface adhesiveness. The differences in adhesiveness of cell lines of high and low malignancy were determined by cell subsets not attaching any Latex particles (24.6–61.9% of cells in highly and 4.6–12.0% in slightly tumorigenic lines) and cell subsets attaching more than 25 Latex particles per cell (14.6–17.2% In highly and 46.1–71.4% in less tumorigenic lines).