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Dissociation of anti‐tumor immune responses in rats immunized with solubilized tumor‐associated antigens from a methylcholanthrene‐induced fibrosarcoma
Author(s) -
Minami Akio,
Mizushima Yutaka,
Takeichi Noritoshi,
Hosokawa Masuo,
Kobayashi Hiroshi
Publication year - 1979
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910230314
Subject(s) - fibrosarcoma , antigen , immune system , methylcholanthrene , spleen , cellular immunity , transplantation , immunity , tumor antigen , immunization , biology , chemistry , microbiology and biotechnology , immunology , immunotherapy , medicine , carcinogen , biochemistry , genetics
Soluble tumor antigens were prepared from chemically‐induced rat fibrosarcoma KMT‐17 cells by various methods [Na‐deoxycholate (DOC), 3 M‐KCI extraction, and crude membrane preparations by mechanical disruption]. Soluble tumor antigens prepared by DOC extraction (DOC‐STA) could be detected by a radioisotopic footpad assay (FPA) and they showed the strongest antigenic activity in KMT‐17 immune rats. Anti‐tumor immune responses in rats previously immunized with DOC‐STA were measured by FPA, Winn assay, and transplantation resistance. Significant responses detected by the FPA and Winn assay were demonstrated in rats immunized with DOC‐STA. However, rats previously immunized with DOC‐STA showed a significant enhancement of tumor growth when challenged with KMT‐17 cells. This enhancement was specific for the tumor line used. Normal rats which received adoptive transfer of thymus and spleen cells from rats immunized with DOC‐STA produced specific enhancement of tumor growth as compared with non‐treated rats. Administration of cyclophosphamide before immunization with DOC‐STA abrogated the enhanced tumor growth in the host. These results suggest that immunization with soluble tumor antigens specifically enhanced tumor growth by the induction of immunosuppressor cells. Dissociation between the anti‐tumor immunity detected by the FPA and Winn assay and the enhanced tumor growth detected by transplantation resistance in rats immunized with DOC‐STA is discussed.