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The metabolic activation of benzo( a )pyrene and 9‐hydroxybenzo ( a ) pyrene by liver microsomal fractions
Author(s) -
Lubet Ronald A.,
Capdevila Jorge,
Prouoh Russell A.
Publication year - 1979
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910230313
Subject(s) - pyrene , microsome , chemistry , biochemistry , environmental chemistry , organic chemistry , enzyme
A rat liver microsome‐mediated bacterial mutagenicity test showed 9‐hydroxybenzo ( a ) pyrene to be significantly more effective as a pre‐mutagen than benzo ( a ) pyrene. Experiments measuring the ability of these compounds to be metabolically activated to moieties that alky late exogenous DNA demonstrated that 9‐hydroxybenzo ( a ) pyrene was almost six times more effective than benzo ( a ) pyrene itself. Addition of trichloropropene‐2,3‐oxide to the reaction mixture enhanced the mutagenicity and DNA alkylation by benzo ( a ) pyrene but had little or no effect on the 9‐hydroxybenzo ( a ) pyrenemediated mutagenicity and alkylation. On the other hand, 7,8‐benzoflavone inhibited the microsome‐mediated mutagenicity and DNA alkylating activity of both hydrocarbons.