Premium
Cell‐density‐dependence for growth in agarose of two human lymphoma lines and its decrease after epstein‐barr virus conversion
Author(s) -
Montaonier L.,
Gruest J.
Publication year - 1979
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910230113
Subject(s) - agarose , plating efficiency , lymphoma , epstein–barr virus , cell culture , virus , transformation (genetics) , biology , virology , cell growth , cell , microbiology and biotechnology , cancer research , immunology , genetics , gene
We have compared the growth in agarose medium of two EBV‐negative Burkitt lymphoma lines, BJAB and Ramos, and that of their EBV‐converted derivatives. Optimal conditions for growth in agarose medium are described. The original EBV‐negative lines can grow in agarose to a high efficiency, provided a critical level of cell concentration is attained. Below this level, there is no growth at all. EBV‐converted sublines form colonies at a much lower cell concentration, and their maximal plating efficiency is higher. These differences may be related to a super‐transformation state induced in these cells by EBV.