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Neoplastic transformation of canine embryo cells in vitro by N‐methyl‐N′‐nitro‐N‐nitrosoguanidine
Author(s) -
Rhim Johng S.,
Park Dai K.,
Arnstein Paul,
NelsonRees Walter A.
Publication year - 1978
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910220412
Subject(s) - in vitro , dimethyl sulfoxide , embryo , beagle , carcinogen , cell culture , transformation (genetics) , methylnitronitrosoguanidine , microbiology and biotechnology , chemistry , biology , andrology , biochemistry , medicine , genetics , organic chemistry , gene , mutant
A cell line derived from a normal beagle embryo was treated in vitro with various levels of N‐methyl‐N′‐nitro‐N‐nitrosoguanidine or dimethyl sulfoxide (control). Cells treated only with the carcinogen underwent morphologic alteration in vitro , and one of these altered cell lines produced tumors sub‐cutaneously when injected into NIH nude mice. The tumorigenic transformed line formed larger cell aggregates and grew in this aggregate form when suspended in liquid growth medium above an agar base.