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Substrate activation of cell adhesion factor as a prerequisite for cell attachment
Author(s) -
Pearlstein Edward
Publication year - 1978
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910220108
Subject(s) - adhesion , cell adhesion , trypsin , cell , substrate (aquarium) , cell culture , biophysics , chemistry , cell–cell interaction , microbiology and biotechnology , monolayer , biochemistry , biology , enzyme , ecology , genetics , organic chemistry
Cell Adhesion Factor, complexed to insoluble collagen‐coated tissue culture dishes, is required for the attachment of fibroblasts to this substrate. In solution, the factor has no demonstrable affinity for cells in suspension following trypsin‐EDTA removal of cells from monolayer. Cell surface receptors for the factor are present during the assay period since cells allowed to recover for 1 h at 37° C, 4° C or in the presence of 10 −6 M cyclo‐heximide show exactly the same kinetics of adhesion as control cells. It is demonstrated that Cell Adhesion Factor acquires affinity for the cell surface only following its binding to collagen.