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Further characterization of a herpesvirus‐positive orang‐utan cell line and comparative aspects of in vitro transformation with lymphotropic old world primate herpesviruses
Author(s) -
Rabin Harvey,
Neubauer Russell H.,
Hopkins Ralph F.,
oyama Meihan
Publication year - 1978
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910210614
Subject(s) - biology , baboon , virology , microbiology and biotechnology , virus , in vitro , genetics , endocrinology
An orang‐utan ( Pongo pygmaeus ) suspension line, CP81, was shown to lack myeloid markers of lysozyme activity and phagocytosis but to be positive for lymphocytic N‐alkaline phosphatase activity, and to release a B‐cell‐tropic herpesvirus. This herpesvirus, termed Herpesvirus pongo , had 30‐40% DNA homology with EBV and was present at 2‐3 genome copies per CP‐81 cell. Gibbon lymphocytes transformed by H. pongo , Epstein‐Barr virus (EBV), and H. papio (of baboon, Papio hamadryas , origin) were found to be virus antigen‐positive B cells. Gibbon lymphocytes transformed by H. pongo and EBV and transplanted to nude mice by the intracranial (IC) route (had a 75% and a 45% success rate, respectively), while transplants of similar cells transformed by H. papio were only 10% successful. None of these lines transplanted subcutaneously (SC) nor manifested a high degree of colony formation in 0.33% agarose (⩽0.5%), Gibbon lymphocytes transformed by H. pongo were hypodiploid while those transformed by EBV or H. papio were diploid. CP‐81 cells themselves could be transplanted both IC (100%) and SC (70%) and showed a relatively high degree of colony formation in agarose (6.4‐7.6%). B95‐8 cells (marmoset, Saguinus oedipus ‐EBV) could be transplanted IC (66%) but not SC and had a low but significant ability to grow in agarose (1.6%).594S (baboon, P. hamadryas ‐H. papio) cells could be transplanted IC (25%) but not SC, and grew to very low levels in agarose (0.1%).