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Search for infective mammalian type‐C virus‐related genes in the dna of human sarcomas and leukemias
Author(s) -
Nicolson M. O.,
McAllister R. M.,
Gilden R. V.,
Charman H.,
Rice N.,
Heberling R.
Publication year - 1978
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910210606
Subject(s) - baboon , virology , biology , virus , leukemia , murine leukemia virus , cell culture , infectivity , dna , embryonic stem cell , microbiology and biotechnology , gene , immunology , genetics , endocrinology
Abstract DNA was extracted from two human sarcoma cell lines, TE‐32 and TE‐418, and the leukemic cells from five children with acute myelocytic leukemia, three children with acute lymphocytic leukemia and four adults with acute myelocytic leukemia. The DNAs, assayed for infectivity by transfection techniques, induced no measurable virus by methods which would detect known mammalian C‐type antigens or RNA‐directed DNA polymerase in TE‐32, D‐17 dog cells and other indicator cells, nor did they recombine with or rescue endogenous human or exogenous murine or baboon type‐C virus. Model systems used as controls were human sarcoma cells, TE‐32 and HT‐1080, and human lymphoma cells, TE‐543, experimentally infected with KiMuLV, GaLV or baboon type‐C virus, all of which released infectious virus and whose DNAs were infectious for TE‐32 and D‐17 dog cells. Other model systems included. two baboon placentas and one embryonic cell strain spontaneously releasing infectious endogenous baboon virus and yielding DNAs infectious for D‐17 dog cells but not for TE‐32 cells. Four other baboon embryonic tissues and two embryonic cell strains, releasing either low levels of virus or no virus, did not yield infectious DNA.