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The complexity of immunohistochemical staining pattern of hodgkin and sternberg‐reed cells—demonstration of immunoglobulin, albumin, α 1 ‐antichymotrypsin and lysozyme
Author(s) -
Papadimitriou Constantin S.,
Stein Harald,
Lennert Karl
Publication year - 1978
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910210502
Subject(s) - immunoperoxidase , immunoglobulin light chain , antibody , pathology , lymph node , immunohistochemistry , alpha chain , lysozyme , microbiology and biotechnology , alpha (finance) , chemistry , biology , immunology , medicine , monoclonal antibody , biochemistry , receptor , construct validity , nursing , patient satisfaction
The immunoperoxidase‐bridge technique was applied to 103 lymph‐node and spleen biopsies from Hodgkin's disease to detect intracytoplasmic immunoglobulin (Clg). The plasma cells that stained positively for gamma, mu, alpha, or delta chains varied greatly in number from case to case, depending somewhat on the histologic type of the disease. Hodgkin (H) and Sternberg‐Reed (SR) cells that stained for gamma chains were the most frequent, followed by those positive for delta chains. H and SR cells positive for mu or alpha chains were usually sparse. In 11 cases gamma and delta chains were present simultaneously in single cells. No other combination of heavy‐chain classes could be found in H and SR cells. In five cases of the lymphocytic‐depletion type, the Clg was restricted to one heavy‐chain class and one light‐chain type. Gamma‐chain subtypes were determined in a total of 24 cases. In the cases with one light‐chain type and in four other cases, only gamma, chains were present in the H and SR cells. In many cases the proportion of H and SR cells positive for each gamma‐chain subclass was not the same as that of the surrounding plasma cells. Variable numbers of H and SR cells showed unequivocal positivity for albumin in 12, alpha 1 ‐antichymotrypsin in 12, and lysozyme in one out of the 44 cases tested. In 21 out of these 44 cases only Clg could be detected. The findings presented here are discussed in relation to the possible monocytoid/macrophage or lymphoid B‐cell nature of H and SR cells. Our data provide evidence for both a lymphatic and a macrophage origin of H and SR cells. It is not possible to reconcile these data on the basis of present knowledge.