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Circulating immune complexes in rats bearing chemically induced tumors. I. Sequential determination during the growth of tumours at various body sites
Author(s) -
Höffken Klaus,
Price Michael R.,
McLaughlin P. Jeremy,
Moore Valerie E.,
Baldwin Robert W.
Publication year - 1978
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910210416
Subject(s) - immune system , immune complex , peritoneal cavity , antigen antibody complex , biology , immunology , cancer research , chemistry , medicine , pathology , anatomy
Circulating immune complexes were measured in sequential monitoring studies in rats bearing chemically induced tumours at different body sites. The assay employed were based upon radio‐immunoprecipitation of serum factors with 125 I‐C1q or with an indirect test whereby the inhibition of binding of 125 I‐C1q to IgG aggregates by tumour‐bearer sera was measured. 125 I‐C1q‐binding immune complexes were detected at the initial phase of tumour growth and the level of this activity returned to the normal range after tumour excision. With tumours growing at subcutaneous sites or in the peritoneal cavity, serum‐borne C1q‐binding material, after reaching a maximum level, decreased despite tumour progression and eventually fell into sub‐normal ranges at terminal stages of growth. In contrast, following intravenous tumour cell injection, immune‐complex levels increased until terminal stages of disease. The present findings indicate that the measurement of C1q‐binding serum factors represents a useful method for monitoring the growth and burden of experimental animal tumours.