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Melphalan‐induced chromosome damage in sensitive and resistant human melanoma cell lines
Author(s) -
Parsons P. G.,
Morrison Leanne
Publication year - 1978
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910210407
Subject(s) - melphalan , cell culture , metaphase , microbiology and biotechnology , melanoma , chromosome , biology , nitrogen mustard , dna , cancer research , chemotherapy , genetics , gene , cyclophosphamide
Abstract Twelve consecutive treatments of a human melanoma cell line (MM253) with melphalan gave a subline (MM253‐12M) which was five times more resistant to melphalan with respect to survival. In contrast to mustard‐resistant rodent cells, the MM253‐12M line had a higher stemline number than the parent line while growth rate and cell and colony morphology were unchanged. A further melphalan treatment following attempted mutagenesis with UV did not increase resistance. In a comparison of these two lines with two melanoma lines derived from other patients and the rat XC line, resistance was correlated with lower frequency of melphalan‐induced chromosome aberrations, determined 48 h after a 4‐h exposure to melphalan(3 μg/ml). In the two cell lines studied, aberration‐free metaphase cells from treated culture had fewer chromosomes than untreated cells. DNA synthesis studied in the 4‐ to 72‐h period after treatment was inhibited to the same extent in MM253 and MM253‐12M cells at 4 μg/ml but to a greater extent in the sensitive line at 0.1‐1.5 μg/ml. During the first hour of treatment at 0.1‐1.5 μg/ml, DNA synthesis in MM253 appeared to be enhanced.