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Detection of circulating tumor antigens in mice carrying a highly metastatic pulmonary squamous‐cell carcinoma
Author(s) -
Jamasbi Roudabeh J.,
Nettesheim Paul,
Kennel Stephen J.
Publication year - 1978
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910210321
Subject(s) - antigen , medicine , basal cell , pathology , carcinoma , cancer research , immunology
Abstract Recently we reported that the highly metastatic MSC‐10 (mouse squamous carcinoma) is incapable of inducing transplantation immunity. Studies reported here were undertaken to determine whether or not the tumor is devoid of tumor‐associated antigen. We found that sera from MSC‐10 tumor‐bearing mice contain soluble protein antigens which react with rabbit antisera made against the MSC‐10 tumor, as demonstrated by immuno‐diffusion. Such proteins were not detected in the sera of normal mice or mice bearing fibrosarcomas. A close temporal relationship was demonstrated between the appearance of circulating antigens and the occurrence of lung metastases. Protein components serologically indistinguishable from the circulating antigens were isolated from tumor cells. The molecular weight of these proteins is between 30,000 and 100,000 daltons. Studies with antisera to mouse leukemia virus showed that the MSC‐10 tumor antigens are not viral proteins. The lack of immunogenicity of this tumor for syngeneic hosts as well as its high metastatic activity may be due to the early appearance of soluble antigens in the circulation.