Isolation of a human prostate carcinoma cell line (DU 145) | Zendy

Stone Kenneth R. | Zendy; Mickey Don D. | Zendy; Wunderli Heidi | Zendy; Mickey George H. | Zendy; Paulson David F. | Zendy

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Isolation of a human prostate carcinoma cell line (DU 145)

Author(s) -

Stone Kenneth R.,

Mickey Don D.,

Wunderli Heidi,

Mickey George H.,

Paulson David F.

Publication year - 1978

Publication title -

international journal of cancer

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.475

H-Index - 234

eISSN - 1097-0215

pISSN - 0020-7136

DOI - 10.1002/ijc.2910210305

Subject(s) - karyotype , biology , cell culture , organelle , tissue culture , chromosome , chromosomal translocation , pathology , microbiology and biotechnology , in vitro , genetics , medicine , gene

A long‐term tissue culture cell line has been derived from a human prostate adenocarcinoma metastatic to the brain. The cell line, DU 145, has been passaged 90 times in vitro over a period of 2 years. The cells are epithelial, grow in isolated islands on plastic Petri dishes, and form colonies in soft agar suspension culture. Karyotypic analysis demonstrates an aneuploid human karyotype with a modal chromosome number of 64. Distinctive marker chromosomes (a translocation Y chromosome, metacentric minute chromosomes and three large acrocentic chromosomes) have been identified. Electron microscopy of the original tumor tissue and of the tissue culture cell line show a remarkable similarity in cell organelle structure.

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