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Induction of ebna precedes the first cellular S‐phase after ebv‐infection of human lymphocytes
Author(s) -
Einhorn Lena,
Ernberg Ingemar
Publication year - 1978
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910210205
Subject(s) - dna synthesis , biology , virus , dna , immunofluorescence , cell division , cell culture , virology , cell , microbiology and biotechnology , cell cycle , indirect immunofluorescence , immunology , antibody , genetics
Using a combination of immunofluorescence and autoradiography, we studied the appearance of EBNA and DNA synthesis in cord‐blood lymphocytes after infection with EBV derived from the B95‐8 cell line. EBNA appeared between 12 and 25 h after addition of the virus. DNA synthesis was detected in EBNA‐positive cells approximately 20 h after the appearance of EBNA. This shows that EBNA induction precedes the first cellular S‐phase and suggests that the cells have not yet entered the cell‐division cycle when EBNA appears. Little, if any, of the total DNA synthesis induced at this stage can be attributed to EBV‐mediated immunologic stimulation.