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Establishment of marek's disease lymphoblastoid cell lines from transplantable versus primary lymphomas
Author(s) -
Calnek B. W.,
Murthy K. K.,
Schat K. A.
Publication year - 1978
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910210117
Subject(s) - marek's disease , lymphoma , cell culture , buffy coat , in vitro , lymphoblast , virology , biology , immunology , virus , genetics
Six new Marek's disease (MD) lymphoblastoid cell lines were established in vitro by cultivation in medium containing 2‐mercaptoethanol (2‐ME). Attempts using primary lymphoma cells were generally unsuccessful; only one of 28 lymphomas yielded a cell line and that one came from an experimentally immunosuppressed chicken. In contrast, two of seven low‐passage, and two of two established MD transplantable lymphomas grew readily in vitro. A sixth line was obtained using buffy coat cells from leukemic chicken. It was concluded that the use of transplantable tumor cells and a medium containing 2‐ME provided a combination highly sulted to the establishment of cell lines from MD.