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Induction of tumor resistance with BCGa‐associated tumor antigen
Author(s) -
Crum E. D.,
McGregor D. D.
Publication year - 1977
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910200521
Subject(s) - antigen , in vitro , tumor antigen , tumor cells , microgram , exudate , suppressor , cancer research , immunization , immunology , microbiology and biotechnology , chemistry , biology , medicine , pathology , cytotoxic t cell , cancer , biochemistry
Soluble material enriched in tumor‐associated antigen was prepared by affinity chromatography from a KCI extract of the chemically‐induced D‐23 rat hepatoma. Microgram quantities of the above material bound spontaneously to living BCG when the two were incubated briefly in vitro . When injected into normal syngeneic rats, the BCG‐associated tumor antigen induced a measure of resistance against challenge with D‐23 tumor cells. Peritoneal exudate cells (PEC) obtained from such actively immunized subjects were able to suppress the growth of D‐23 tumor cells at a test site in muscle. In contrast, immunization with either BCG alone, tumor protein alone, or tumor protein admixed with BCG in circumstances designed to impede association of the protein, failed to provoke the formation of tumor suppressor PEC. The results encourage the belief that binding of tumor antigen to BCG favors the induction of a cell‐mediated tumor suppressive response.