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Glycopeptides from epithelial cell mutants: Temperature sensitive for the transformation phenotype
Author(s) -
Pietropaolo Concetta,
Yamaguchi Nobuo,
Weinstein I. Bernard,
Click Mary Catherine
Publication year - 1977
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910200514
Subject(s) - glycopeptide , sephadex , size exclusion chromatography , cell , phenotype , in vitro , mutant , biology , cell culture , fucose , glycoprotein , biochemistry , chemistry , microbiology and biotechnology , gene , enzyme , genetics , antibiotics
Fucose‐labelled glycopeptides obtained from the cell surfaces of normal and transformed epithelial cells were compared by co‐chromatography on Sephadex G‐5O. The material obtained from rat epithelial cells transformed in vitro or from hepatoma cells in culture elutes earlier than the fucosecontaining glycopeptides obtained from normal rat epithelial cells. A mutant (TS 223) of a transformed epithelial cell that is temperaturesensitive for maintenance of the transformed phenotype, varies in its Sephadex G‐50 profile of cell surface glycopeptides when grown at the permissive (34° C) or the non‐permissive temperature (40° C). When grown and labelled at 36° C the gel filtration profile of the glycopeptides resembles that of transformed cells. At 40° C there is an enrichment of later eluting glycopeptides. These differences are more striking in confluent‐phase cultures than in log‐phase cultures. The differences are reversible following upward or downward shifts in growth temperature although there appears to be a lag of at least 6 h before the alteration can be demonstrated by these procedures.