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Growth inhibitory effects of interferon on normal and malignant human haemopoietic cells
Author(s) -
Balkwill Frances R.,
Oliver R. T. D.
Publication year - 1977
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910200405
Subject(s) - lymphoblast , interferon , thymidine , bone marrow , lymphokine , interferon alfa , alpha interferon , cell culture , cell growth , cell , immune system , biology , immunology , medicine , in vitro , biochemistry , genetics
A study of the effects of human leukocyte and lymphoblastoid interferon preparations on the growth of normal, immune and malignant haemopoietic cells has been carried out. At a standard dose of 10,000 U/ml, incorporation of tritiated thymidine ([ 3 H] TdR) was reduced by 7–92% of control values, and cell survival by 35–82% in acute myelogenous leukaemia cell cultures, whereas in normal bone‐marrow cultures interferon showed a 58–62% reduction in [ 3 H] TdR uptake but only up to 13% reduction in cell survival. [ 3 H] TdR incorporation by MLC‐stimulated lymphocytes was also significantly reduced by interferon but the blastogenic response to PHA was not. These effects of interferon were shown to be dose‐dependent. The problems of using interferon in the treatment of AML in the light of these findings are discussed.