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Detection of epstein‐barr virus antigens with enzyme‐conjugated antibody
Author(s) -
Granlund David J.,
Andrese Angelo P.
Publication year - 1977
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910200404
Subject(s) - antigen , antibody , virus , conjugated system , epstein–barr virus , virology , enzyme , biology , medicine , immunology , chemistry , biochemistry , organic chemistry , polymer
Monospecific conjugated (fluorescein isothiocynate and horseradish peroxidase) goat antisera, prepared against three human immunoglobulin classes, IgM (μ), IgG (γ) and IgA (α), were compared for their ability to detect human Ig classes possessing specificity for Epstein‐Barr virus (EBV) viral capsid antigens (VCA) in a chronically infected human lymphoid cell line, P3J‐HRIK. It was determined that the enzyme system was significantly more sensitive than immunofluorescence in detecting most of the immunoglobulins in sera from cancer patients. Some patients with nasopharyngeal carcinomas (NPC) had extremely high levels of EBV‐specific IgA, whereas cancers other than NPC may have lower EBV‐specific IgA titers.