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Delayed hypersensitivity in tumor‐bearing mice. In vitro activation of “eclipsed” spleen cells
Author(s) -
Jasnis Maria A.,
Klein Slobodanka,
de Bonaparte Yolanda P.,
de Daga Sara O.,
de Pirosky Rosa R.
Publication year - 1977
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910200311
Subject(s) - in vivo , in vitro , spleen , delayed hypersensitivity , immune system , antigen , immunology , pathology , biology , medicine , biochemistry , microbiology and biotechnology
At various stages during the progressive growth of a transplanted sarcoma in BALB/c mice, the delayed hypersensitivity response to tumor antigen was determined using the food‐pad swelling test (FPS) and the leukocyte migration inhibition assay (LMI). A close correlation was observed between the in vivo and in vitro assays. “Early” recognition of tumor antigen was detected 24 h after tumor inoculation by both techniques and this positive response was maintained until day 15. As the tumor grew larger, the delayed hypersensitivity response in vivo vanished, while the delayed hypersensitivity response in vitro disappeared about 3 days later. This suppression or “eclipse” of the anti‐tumor cellular immune response was specific for the type of tumor used, and could be reversed in vitro by means of a low pH treatment of lymphoid cells.