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Detection of herpes simplex virus‐related antigens in the nuclei and cytoplasm of biochemically transformed cells with peroxidase/anti‐peroxidase immunological staining and indirect immunofluorescence
Author(s) -
Kurchak Mark,
Dubbs Del Rose,
Kit Saul
Publication year - 1977
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910200308
Subject(s) - peroxidase , immunofluorescence , cytoplasm , antigen , staining , virology , herpes simplex virus , indirect immunofluorescence , biology , virus , antibody , enzyme , immunology , biochemistry , genetics
Herpes simplex virus (HSV)‐related antigens have been demonstrated in the nuclei and cytoplasm of human and mouse cells biochemically transformed by ultraviolet light‐irradiated HSV. This was accomplished by using peroxidase/anti‐peroxidase immunological staining and indirect immunofluorescence with rabbit antisera that had high neutralizing titers against the HSV‐specific thymidine kinase activity and virus infectivity. HSV‐1 antisera reacted with antigens in cells biochemically transformed by type 1 HSV, but not with those of cells biochemically transformed by type 2 HSV. Similarly, HSV‐2 antisera reacted with antigens in cells biochemically transformed by HSV‐2, but not with those in cells biochemically transformed by HSV‐1. In contrast, herpes virus‐related antigens were detected in cells cytolytically infected with HSV‐1 and with HSV‐2 by either type 1 or type 2 HSV antisera. These observations suggest that the antigens detected in the biochemically transformed cells were a type‐specific subset of the HSV‐related antigens synthesized in cells undergoing productive infection by HSV‐1 and HSV‐2.