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Expression of alien h‐2 specificities on a chemically induced balb/c fibrosarcoma
Author(s) -
Meschini Angela,
Invernizzi Giovanni,
Parmiani Giorgio
Publication year - 1977
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910200216
Subject(s) - fibrosarcoma , antiserum , antigen , microbiology and biotechnology , transplantation , histocompatibility , biology , balb/c , major histocompatibility complex , serology , immunology , antibody , immune system , medicine , human leukocyte antigen , genetics , surgery
Abstract The presence of alien histocompatibility antigens on the cell surface of the 3‐methylcholanthreneinduced BALB/c (H‐2 d ) fibrosarcoma C‐1, was investigated by serological and transplantation studies. Absorption experiments with monospecific alloantisera showed that C‐1 cells expressed their original private (H‐2.4 and 31) and public (H‐2.3, 8, 28, and 35) specificities. C‐1 cells were also able to absorb monospecific antisera directed to the private specificity H‐2.23 of the H‐2 k haplotype, as well as antisera to the public specificities H‐2.1, 5, 11 and 25 (H‐2 k and in part H‐2 q , H‐2 a and H‐2 b haplotypes), which are absent from H‐2 d normal cells. Conversely, other alien specificities (H‐2.2, 17, 30, 32, and 33) were not detected on C‐1 cells. The C‐1 cells were also unable to absorb the activity of an anti‐la serum (1–28) directed to la.1, 2 and 19 (la k ) specificities. Transplantation studies showed that resistance against the challenge of C‐1 cells could be induced in syngeneic BALB/c mice by preimmunization with normal tissues from C3Hf and AKR (H‐2 k ), A (H‐2 a ) and C57BL/6J (H‐2 b ) strains (expressing all or some of the extra H‐2 antigens of the tumor) whereas no protection was obtained with DBA/2 (H‐2 d ) or with W/Fu rat tissues. The anti‐tumor activity could be passively transferred by BALB/c lymphoid cells immune to normal C3Hf, AKR, A, and NIH (H‐2 q ) tissues, but no protection was achieved with lymphoid cells immune to DBA/2 or to W/Fu normal rat tissues. These data indicate that foreign H‐2 antigens are expressed on C‐1 tumor and that they might function as tumor‐associated transplantation antigen which was shown to be present and individually distinct on this sarcoma by appropriate in vivo tests.