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Establishment of simian sarcoma virus, type 1 (SSV‐1)‐transformed non‐producer marmoset cell lines
Author(s) -
Bergholz Carolyn M.,
Wolfe Lauren G.,
Deinhardt Friedrich
Publication year - 1977
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910200117
Subject(s) - biology , cell culture , virus , virology , marmoset , immunofluorescence , fibrosarcoma , cell , microbiology and biotechnology , fibroblast , antibody , immunology , genetics , paleontology
Simian sarcoma virus, type 1 (SSV‐1)‐transformed non‐producer cell lines were established by infection of normal marmoset fibroblast cells (HF) with limiting dilutions of SSV‐1. Four focus‐derived cell lines were identified as non‐producers by assay of culture fluids for focus‐forming activity and by the mixed culture cytopathogenicity test with XC cells. Further studies failed to detect production of type‐C virus by 3 H‐uridine labelling, reverse transcriptase assay or electron microscopy. The non‐producer cell lines, designated HF/SSV‐NPI, IV, V, and VI, appeared morphologically transformed and cloned in soft agar with the same efficiency as HF/SSV‐1 virus‐producing transformed cells. Expression of either cytoplasmic or cell surface virus‐related antingens was not detected by immunofluorescence or serum cytotoxicity tests. The presence of sarcoma genome in the transformed non‐producer cell lines was demonstrated by rescue of focus‐forming activity following superinfection with non‐transforming helper virus or by cocultivation with helper‐virus‐producing cell lines. The SSV‐1‐transformed non‐producer primate cells provide a useful tool for future studies.