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Drug sensitivity studies on clonal cell lines isolated from heteroploid tumour cell populations. I. Dose response of clones growing in monolayer cultures
Author(s) -
Pályi I.,
Oláh Edit,
Sugár J.
Publication year - 1977
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910190618
Subject(s) - vinca , clone (java method) , cell culture , biology , vincristine , microbiology and biotechnology , cell , tissue culture , cell division , chemotherapy , genetics , dna , in vitro , pharmacology , cyclophosphamide
Drug sensitivity of a heteroploid tumour cell line and three clones isolated from it was studied in tissue culture. Cells of the parent line, P 0 , and of clone P 1 were fibroblast‐like and pseudo‐ and hypodiploid. Clones P 3 and P 4 consisted mostly of epithelial and round cells, respectively, and were hypotetraploid. In dose‐response experiments, P 1 and P 4 clones were more sensitive to dianhydro‐dulcitol than P 0 or P 3 clones. Adriamycin was more toxic to P 3 than to the other three cell lines. P 3 and P 4 clones were more sensitive to vincristine than P 0 or P 1 clones. N‐formyl‐leurosine, a new derivative of the Vinca alkaloids, was least toxic to P 4 cells, and the other three cell lines were equally sensitive. It was concluded that relative sensitivity of the clones depended on the particular drug. Presence of clones of lower sensitivity in heteroploid cell populations may have some bearing on regrowth of tumours after chemotherapy.