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Incomplete viral genome in a non‐virogenic mouse tumour cell line (RVP 3 ) transformed by prague strain of avian sarcoma virus
Author(s) -
Svoboda J.,
Popovič M.,
Sainerová H.,
Mach O.,
Shoyab M.,
Baluda M. A.
Publication year - 1977
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910190617
Subject(s) - rous sarcoma virus , virology , genome , biology , virus , cell culture , strain (injury) , cell fusion , transfection , gene , microbiology and biotechnology , genetics , anatomy
Two cell lines, RVP 3 and RVA 4 , derived originally from mouse tumours induced by the Prague and Schmidt‐Ruppin strain of RSV, respectively, were studied. All attempts failed to induce infectious virus production in RVP 3 cells by fusion with chicken fibroblasts even if the cells were infected with avian leukosis viruses. Also, attempts to rescue the viral genome by transfection were unsuccessful. RVP 3 cells harboured 31–45% of the viral genome sequences, as was shown by molecular hybridization, and therefore they were designated cryptovirogenic. The tumour cell line RVA 4 did not contain any detectable viral sequences. The significance of the detection of the incomplete Rous virus genome sequences in mammalian cells is discussed.