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Immunologic responses to a murine mammary adenocarcinoma. I. Passive transfer of immunity by sera from tumor‐bearing mice
Author(s) -
Yamamura Yasuhiro,
Virella G.,
Haskill J. S.
Publication year - 1977
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910190517
Subject(s) - fibrosarcoma , immune system , antibody , immunology , immunity , titer , mammary tumor , humoral immunity , immunofluorescence , antibody titer , biology , passive immunity , medicine , cancer , pathology , breast cancer
DBA/2 mice bearing a syngeneic mammary adenocarcioma T1699 produced high levels of tumor‐specific antibody, detected by indirect immunofluorescence and subsequently identified as the IgG 2a subclass. Tumor‐bearer sera passively administered to normal recipients protected the animals from subsequent challenge with T1699 tumor cells but not from challenge with a non‐cross‐reacting syngeneic tumor, SaD 2 fibrosarcoma. Administration of sera prior to tumor challenge was found to be more effective than treatment after the challenge. The protective effect of sera appeared to parallel both antibody titers and appearance of concomitant immunity; however, sera absorbed with T1699 cells, with the indirect fluorescent antibody titers reduced more than 100‐fold, conferred an almost identical level of protection. Immune suppression of serum recipients before serum transfer abolished the effect, suggesting that protection depended on a cellular immune response by the host in addition to the possible protective effect(s) of humoral antibody.