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Detection of SV40 T antigen with labelled antibodies: Radioimmunoassay and autoradiography
Author(s) -
Flügel R. M.,
Crefeld T.,
Munk K.
Publication year - 1977
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910190509
Subject(s) - radioimmunoassay , antigen , hamster , immunofluorescence , microbiology and biotechnology , antibody , chemistry , biology , chromatography , immunology , biochemistry
SV40 T antigen, partially purified from SV80, a human cell line, has been used to develop a sensitive solid phase radioimmunoassay. In this assay SV40 T antigen was immobilized on microtiter plates, reacted with hamster anti‐SV40 T antibody and subsequently with 14 C‐labelled anti‐hamster IgG. The test detected approximately 20 ng of partially purified SV40 T antigen and is rapid and more convenient than the complement fixing reaction. An immunoautoradiographic method which permits the determination of the cellular localization of SV40 T antigen and which is possibly more sensitive than the indirect immunofluorescence method is also described.