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Induction by cigarette smoke of aryl hydrocarbon hydroxylase activity in the rat kidney and lung 1
Author(s) -
Van Cantfort Jacques,
Gielen Jacques
Publication year - 1977
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910190415
Subject(s) - kidney , lung , enzyme , chemistry , inhalation , smoke , cigarette smoke , enzyme assay , medicine , endocrinology , in vitro , in vivo , biochemistry , biology , toxicology , anatomy , organic chemistry , microbiology and biotechnology
In both the rat kidney and lung, inhalation of cigarette smoke diluted with air (1/15) for a limited period of time (15 min) specifically induces aryl hydrocarbon hydroxylase (AHH)<2> in less than 4 h. Up to four successive inhalations administered at 2‐h intervals additively induce both lung and kidney hydroxylase activities. The maximal effect corresponds to about 10 times the control value. Compared to the kidney enzyme, the lung AHH activity is about three or four times more sensitive to small concentrations of cigarette smoke. The biological half‐life of the lung AHH activity is longer than 24 h, while it is only 3–4 h in the kidney. In both tissues, the induced enzyme presents the same in vitro thermolability and sensitivity to various inhibitors. For the establishment of the AHH induction, protein synthesis is continuously required, while RNA synthesis is only necessary during the first 2 h following the smoke treatment.