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Isolation from a transmissible lymphoid tumour (tlt) lymphoblastoid cell line of a herpesvirus similar to marek's disease virus
Author(s) -
Nazerian K.,
Lee Lucy F.,
Witter R. L.
Publication year - 1977
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910190318
Subject(s) - marek's disease , virology , virus , biology , antigen , herpesviridae , lymphoblast , cell culture , lymphoma , immunology , viral disease , genetics
A chicken lymphoblastoid cell line (TLT)‐6855 originally established from an avian oncornavirus‐induced lymphoma (Siegfried and Olson, 1972) was studied for the presence and expression of Marek's disease virus (MDV) genome. By nucleic acid hybridization a significant amount of MDV DNA was found in this cell line. This virus DNA, however, was not expressed in either virus‐specific intracellular or membrane antigens or the MD‐associated tumour‐specific surface antigen (MATSA). Moreover, MDV‐specific antigens could not be activated in this cell line by treatment with 5‐IdUrd. In several experiments, when chickens were inoculated with the cell line a herpesvirus was repeatedly isolated from the kidneys. This herpesvirus was antigenically similar to MDV but was low in oncogenicity for chickens.