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Transformation of rabbit kidney cells by bkv(mm) human papovavirus
Author(s) -
Mason David H.,
Takemoto Kenneth K.
Publication year - 1977
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910190317
Subject(s) - papovavirus , bk virus , antiserum , antigen , biology , virology , kidney , microbiology and biotechnology , virus , immunology , kidney transplantation , endocrinology
Primary rabbit kidney cells were transformed by BKV(MM), a papovavirus isolated from the urine of a child with the Wiskott‐Aldrich syndrome. The transformed cells contained BK T‐antigen, but no antigen that reacted with SV40 U‐antiserum. The transformed cells failed to produce tumors in nude mice, and BKV (MM) was not rescued from transformed cells by cell fusion or chemical induction methods. The transformed cells supported the growth of rabbit kidney vacuolating virus (RKV), and could be used to quantitate RKV by plaque formation under an agar overlay.