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Comparative studies on ebv antigens by immuno‐fluorescence and immunoperoxidase techniques
Author(s) -
Stephens R.,
Traul K.,
Gaudreau P.,
Yeh J.,
Fisher L.,
Mayyasi S. A.
Publication year - 1977
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910190305
Subject(s) - antigen , immunoperoxidase , raji cell , virology , antibody , immunocytochemistry , biology , microbiology and biotechnology , immunology , fluorescence , chemistry , monoclonal antibody , pathology , medicine , physics , quantum mechanics
Abstract Three groups of EBV antigens, VCA, MA and EA, were compared by the techniques of electron microscopic immunoperoxidase (IP) and immuno‐fluorescence (IF). P3HR‐1 and EBV superinfected Raji cells served as targets for labelled sera from patients with BL, NPC and IM or from healthy donors. 125 I peroxidase‐labelled antibodies were also prepared to determine, autoradiographically, the penetration of the complex into the cell system, and to monitor the incubation and washing procedures. The development of a gentle sedimentation technique proved critical in handling the fragile target cells. VCA and MA were readily identified and localized by both procedures without significant modification of the basic techniques. Indentification of early antigens by IP required modification of the fixation method to include a brief treatment in acetone. The diffuse early antigen (EAD) was found to be associated with cellular ribosomes.