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Specificities in natural cell‐mediated cytotoxicity by the cross‐competition assay
Author(s) -
Takasugi M.,
Koide Y.,
Akira D.,
Ramseyer A.
Publication year - 1977
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910190303
Subject(s) - cytotoxicity , effector , antigen , biology , cell , cytotoxic t cell , cell culture , microbiology and biotechnology , chemistry , immunology , biochemistry , in vitro , genetics
The cross‐competition assay was applied to natural cell‐mediated cytotoxicity in human, mouse and rat systems. The results showed that what appeared to be non‐selective effects of natural cytotoxicity were in fact highly specific. Each effector suspension included natural effector cells (N cells) that recognized and reacted specifically with many different antigens on target cells, resulting in overall non‐selectivity. The specificity of the reaction for each target cell was demonstrated by the selective inhibition of natural cytotoxicity achieved when competitor cells sharing antigens with the target cell were added. Sharing of common target antigens was demonstrated by selective cross‐inhibition which was then used to investigate antigens on target cells.