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Polyoma genome transcription in transformed mouse cells growing in culture and as tumors in syngeneic mice
Author(s) -
Grady Leo J.,
North Arlene B.,
Campbell Wayne P.
Publication year - 1977
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910190213
Subject(s) - genome , biology , transcription (linguistics) , neoplasm , cancer research , gene , microbiology and biotechnology , genetics , linguistics , philosophy
Abstract The strands of the polyoma genome coding for early and late viral RNA were separated by means of asymmetric cRNA synthesized under high salt conditions by Escherichia coli RNA polymerase. Each strand was then employed in RNA‐DNA hybridization experiments to determine the degree to which it is transcribed in transformed cells under several conditions. Except for a concanavalin‐A‐selected revertant, approximately one‐quarter of the early strand was expressed in all of the situations investigated. In contrast, while no significant late strand transcription was detected in transformed cells in culture, the tumors induced by these cells contained transcripts complementary to about 12% of this strand. The results are discussed in terms of current knowledge of the amount of the virus genome required to transform cells.