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Human pancreatic carcinoma (mia paca‐2) in continuous culture: Sensitivity to asparaginase
Author(s) -
Yunis Adel A.,
Arimura Grace K.,
Russin David J.
Publication year - 1977
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910190118
Subject(s) - cell culture , fetal bovine serum , pancreatic carcinoma , biology , cytoplasm , carcinoma , aneuploidy , fetus , microbiology and biotechnology , pathology , endocrinology , pancreatic cancer , cancer , medicine , biochemistry , genetics , pregnancy , gene , chromosome
An undifferentiated human pancreatic carcinoma has been established in continuous culture and is grown in Dulbecco's modified Eagle's medium fortified with 10% fetal calf serum and 2.5% horse serum. The established cell line (MIA PaCa‐2) has a doulbing time of 40 h. The cells are large with abundant cytoplasm, exhibit a high degree of aneuploidy and have a tendency to grow on top of other cells. MIA PaCa‐2 grows in soft agar with a colony‐forming efficiency of 19%. Both MIA PaCa‐2 cells and a cell line from another pancreatic carcinoma obtained from National Cancer Institute (NCI) are sensitive to asparaginase, a property not shared by several other human tumor cell lines tested.