Human pancreatic carcinoma (mia paca‐2) in continuous culture: Sensitivity to asparaginase | Zendy

Yunis Adel A. | Zendy; Arimura Grace K. | Zendy; Russin David J. | Zendy

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Human pancreatic carcinoma (mia paca‐2) in continuous culture: Sensitivity to asparaginase

Author(s) -

Yunis Adel A.,

Arimura Grace K.,

Russin David J.

Publication year - 1977

Publication title -

international journal of cancer

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.475

H-Index - 234

eISSN - 1097-0215

pISSN - 0020-7136

DOI - 10.1002/ijc.2910190118

Subject(s) - cell culture , fetal bovine serum , pancreatic carcinoma , biology , cytoplasm , carcinoma , aneuploidy , fetus , microbiology and biotechnology , pathology , endocrinology , pancreatic cancer , cancer , medicine , biochemistry , genetics , pregnancy , gene , chromosome

An undifferentiated human pancreatic carcinoma has been established in continuous culture and is grown in Dulbecco's modified Eagle's medium fortified with 10% fetal calf serum and 2.5% horse serum. The established cell line (MIA PaCa‐2) has a doulbing time of 40 h. The cells are large with abundant cytoplasm, exhibit a high degree of aneuploidy and have a tendency to grow on top of other cells. MIA PaCa‐2 grows in soft agar with a colony‐forming efficiency of 19%. Both MIA PaCa‐2 cells and a cell line from another pancreatic carcinoma obtained from National Cancer Institute (NCI) are sensitive to asparaginase, a property not shared by several other human tumor cell lines tested.

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