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Expression of feline type‐C virus in normal and tumor tissues of the domestic cat
Author(s) -
Levin Reuven,
Ruscetti Sandra K.,
Parks Wade P.,
Scolnick Edward M.
Publication year - 1976
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910180515
Subject(s) - biology , virus , nucleic acid , dna , virology , cats , rna , feline leukemia virus , homologous chromosome , microbiology and biotechnology , antigen , gene , immunology , genetics , medicine
Tumor and normal tissues from domestic cats were examined for FeLV and RD‐114 specific nucleic acids and proteins. Virus‐positive tissues showed a 100‐ to 200‐fold higher level of FeLV and a 200‐ to 400‐fold higher level of FeLV p30 than virus‐negative tumors and normal cat tissues. In contrast, the levels of RD‐114 viral RNA and p30 antigen were comparable in the majority of tumorous and normal tissues examined. When the DNA of these tissues was examined for FeLV‐related sequences, the virus‐positive tumors were found to contain extra copies of FeLV DNA. Virus‐negative tumors contained DNA sequences homologous with FeLV but the copy number of these sequences could not be distinguished from normal tissues. The results indicate that in certain FeLV‐induced type‐C lymphoproliferative diseases, extra type‐C sequences not present in normal tissues can be identified.