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Cell surface changes observed in mc‐29 virus‐infected chicken‐embryo fibroblast (cec) cultures
Author(s) -
Podmaniczky Erzsebet,
Szende B.,
Lapis K.,
Ferenc G.
Publication year - 1976
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910180419
Subject(s) - virus , budding , vacuole , intracellular , virology , embryo , biology , fibroblast , cell culture , electron microscope , scanning electron microscope , microbiology and biotechnology , cytoplasm , materials science , genetics , physics , optics , composite material
By transmission and scanning electron microscopic studies of MC‐29 virus‐infected CEC cultures, C‐type virus particles were detected in the medium of infected cultures, in infected cells and in intracellular vacuoles. Virus budding was also observed. In the scanning electron microscope (SEM) so‐called blebs appeared on the surfaces of the infected cells, in a number proportional to the concentration of the virus preparation. On the basis of the size of the blebs, it could be considered that virus formation was taking place in their center. In control cells, neither viruses nor blebs were observed. This method will presumably be suitable for the titration of virus preparations.