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Surface glycoproteins and concanavalin‐A‐mediated agglutinability of clonal variants and tumour cells derived from SV40‐virus‐transformed mouse 3T3 cells
Author(s) -
Smets L. A.,
Van Beek W. P.,
Van Rooij H.
Publication year - 1976
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910180411
Subject(s) - concanavalin a , 3t3 cells , glycoprotein , cell , cell culture , phenotype , in vitro , biology , cell division , fucose , microbiology and biotechnology , biochemistry , transfection , gene , genetics
Cell strains isolated from an established line of SV40‐transformed mouse 3T3 cells (SV3T3) showed large variations in the various parameters of transformation, viz. saturation density, serum requirement, contact inhibition of movement and of growth and Concanavalin‐A‐mediated agglutinability. These cell strains were studied for changes in elution profiles of fucose‐labelled surface glycoproteins, using actively growing, untransformed 3T3 cells as controls. A cell strain established from a tumour arising after injection of wild‐type SV3T3 cells and SV3T3 cells grown in vitro in diffusion chambers, was similarly studied. Changes in surface glycoproteins were observed only in the tumour‐derived cell strain. The results suggest that alterations in surface glycoproteins are associated with the tumorigenic potential of the cells rather than with the transformed phenotype per se.