Premium
F c receptor‐bearing cells as a reliable marker for quantitation of host lymphoreticular infiltration of progressively growing solid tumors
Author(s) -
Kerbel R. S.,
Pross H. F.
Publication year - 1976
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910180406
Subject(s) - fibrosarcoma , receptor , pathology , infiltration (hvac) , cell , biology , cancer research , medicine , biochemistry , physics , thermodynamics
Disaggregated cell suspensions made from transplanted solid tumors, either chemically‐induced fibrosarcomas, or spontaneous mammary carcinomas, can contain very high numbers of F c receptor‐bearing cells which are of host origin. Because most types of lymphoreticular cells have F c receptors, and because T cells—most of which are F c receptor‐negative—appear to infiltrate such tumors only to a very limited degree, the possibility that F c receptor cells could serve as a reliable and simple marker for host lymphoreticular cell infiltration of solid tumors was tested. This was accomplished by comparing the ratios of F c rosetting cells to serologically detectable host cells in H2 d or H2 k haplotype tumor cell suspensions grown in (H2 d × H2 k ) F 1 hybrid mice, where host cells could be distinguished from tumor cells by treatment with the appropriate anti‐H2 serum. Ratios of 0.8 to 1.08 were obtained for four different tumors including the SaD/2 fibrosarcoma, a CBA spontaneous fibrosarcoma, and the T1699 and CaD/2 mammary carcinomas. Analysis of the results showed that enumeration of F c rosettes was a reliable host cell marker for at least three of the four tumors tested. The mean non‐malignant host cell content of the various tumors, as assessed by anti‐H2 cytotoxicity tests, ranged from 23% to 41%.