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Production of tumor‐specific inducer of cellular cytotoxicity by lethally irradiated mice
Author(s) -
Pollack Sylvia B.,
Nelson Karen
Publication year - 1976
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910180216
Subject(s) - cytotoxicity , spleen , antiserum , sarcoma , microbiology and biotechnology , immunology , biology , in vitro , cell , antibody , pathology , cancer research , medicine , biochemistry
Antisera taken 1 or 2 days after inoculation of BALB/c mice with transplantable sarcoma cells or Moloney sarcoma virus (MSV) induce tumor‐specific cell‐dependent cytotoxicity in vitro . In the present experiments, lethally irradiated (“immunosup‐pressed”) mice were tested for the early appearance of the serum factor responsible for this anti‐serum dependent cell‐mediated cytotoxicity (E‐ADC). BALB/c mice were injected with MSV, syngeneic sarcoma cells or sheep red blood cells 24 h following irradiation with 900 R. Sera were obtained from MSV and tumor cell recipients 48 or 72 h later and tested for E‐ADC activity. Spleen cells from SRBC recipients were tested at day 4 or 5 for ability to form direct plaques in a modified Jerne plaque assay. Although the anti‐SRBC response was obliterated in the irradiated mice, the E‐ADC response appeared to be unimpaired. These studies indicate that newly synthesized immunoglobulin is not required for the formation of the E‐ADC factor.