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Isolation from human gastric juice of an antigen closely related to the carcinoembryonic antigen
Author(s) -
Vuento Matti,
Engvall Eva,
Seppälä Markku,
Ruoslahti Erkki
Publication year - 1976
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910180204
Subject(s) - carcinoembryonic antigen , antigen , isolation (microbiology) , medicine , immunology , biology , microbiology and biotechnology , cancer
A carcinoembryonic antigen‐like antigen(s) (CELIA) was isolated from normal human gastric juice by immunoadsorbents and gel chromatography. The isolated CELIA reacted identically to CEA in immunodiffusion tests against anti‐CEA and anti‐CELIA. Other experiments suggested subtle differences between CELIA and CEA. Two out of six CELIA preparations tested in immunodiffusion did not react in a completely identical manner with CEA. In gel filtration, CELIA eluted slightly after CEA. Absorption of anti‐CEA with CELIA resulted in antisera with greatly reduced reactivity to CELIA, but retained anti‐CEA activity. Such antisera may be helpful in attempts to increase the cancer specificity of the CEA test.