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Immunogenic and immunosuppressive components in soluble preparations from moloney murine leukemia virus‐induced tumor cells
Author(s) -
Brandchaft Phyllis B.,
Aoki Tadao,
Silverman Toby N.
Publication year - 1976
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910170518
Subject(s) - murine leukemia virus , leukemia , cytotoxic t cell , neoplasm , sephadex , biology , antibody , virus , immune system , immunology , virology , microbiology and biotechnology , chemistry , biochemistry , in vitro , enzyme , genetics
Soluble extracts from Moloney virus‐induced leukemia cells were shown to contain immunogenic and immunosuppressive components. Immunogenic components were divided into two classes based on their ability to: (1) combine with humoral antibody to inhibit cytotoxic activity; or (2) stimulate blastogenesis in normal syngeneic lymphocytes. Immunosuppressive components were demonstrated by the inhibition of DNA synthesis in normal syngeneic lymphocytes and suppression of a mixed lymphocyte reaction. Stimulating components in many soluble preparations were completely masked by immunosuppressive components. However, all three components could be resolved by Sephadex G200 chromatography.