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The detection of virus DNA sequences in a herpes type 2 transformed hamster cell line (333‐8‐9)
Author(s) -
Minson A. C.,
Thouless M. E.,
Eglin R. P.,
Darby G.
Publication year - 1976
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910170412
Subject(s) - dna , hamster , virus , herpes simplex virus , virology , biology , cell culture , genome , dna sequencing , microbiology and biotechnology , in vitro , genetics , gene
Herpes simplex virus type 2 DNA labelled in vitro with 125 I has been used as a probe to search for virus DNA sequences in the 333‐8‐9 line of transformed primary hamster cells and in clones derived from the line. Virus DNA sequences were present when the cells were examined initially but these sequences were lost on subsequent handling. No virus sequences were detected in clones derived from the line. It is suggested that the presence of virus DNA is an unstable characteristic of the cell line and that these sequences may be lost at cell division. The possibility that a small fragment of the genome remains cannot be excluded.