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Mixed lymphocyte/tumor‐cell interaction in a murine sarcoma virus (moloney)‐induced tumor system. Comparison between lymphoproliferation and lymphocyte cytotoxicity
Author(s) -
Kirchner Holger,
Glaser Moshe,
Holden Howard T.,
Herberman Ronald B.
Publication year - 1976
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910170313
Subject(s) - cytotoxicity , in vivo , cytotoxic t cell , lymphocyte , spleen , in vitro , immunology , neoplasm , transplantation , biology , microbiology and biotechnology , chemistry , cancer research , pathology , medicine , biochemistry
Abstract Studies of the MLTI were performed in a primary MSV‐induced tumor system. Reactivity against mitomycin‐C‐treated RBL‐5 cells could be detected during a limited period at about 14 days after virus injection. The degree of reactivity of MSV 14 spleen cells was quite variable; stimulation indices ranged from 2.2 to 9.4. Activity in the MLTI appeared to a great extent to be dependent on T‐lymphocytes. A relatively broad spectrum of specificity was demonstrated in experiments using additional stimulating cells besides RBL‐5. The kinetics of the MLTI correlated well with the kinetics of the primary cytotoxic response in the CRA against RBL‐5 cells. However, positive results in the in vivo and in vitro secondary cytotoxicity assays could be detected between 21 and 100 days after MSV injection when the MLTI was negative. Transplantation resistance against challenge with RBL‐5 tumor cells also persists for a long time after MSV inoculation. Our data therefore indicate that the MLTI, in contrast to the secondary cytotoxicity assays, does not correlate well with the status of in vivo immunity.