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Incomplete viral synthesis in friend leukemia virusinduced reticulum cell sarcomas
Author(s) -
Black Douglas R.,
Fieldsteel A. Howard
Publication year - 1976
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910170213
Subject(s) - cell culture , virology , biology , uridine , leukemia , murine leukemia virus , nucleic acid , cell , virus , rna , microbiology and biotechnology , reverse transcriptase , immunology , genetics , gene
Tissue‐culture‐passaged, Friend leukemia virus (FV)‐induced reticulum cell sarcomas from BALB/c mice (FVTCT‐BALB) did not produce infectious FV, although retrieval of infectious FV occurred when these cells were co‐cultivated with cell lines replicating non‐defective murine leukemia viruses (MLVs). The level of FV expression in the FVTCT‐BALB cell line was studied to understand better the process of FV retrieval. 3 H‐uridine labelling techniques and reverse transcriptase assays showed that FVTCT‐BALB cells did not release C‐type virus particles. Nucleic acid hybridization techniques demonstrated that the level of viral RNA synthesis in the FVTCT‐BALB non‐producer cell line was indistinguishable from that in cell lines productively infected with MLVs. These data suggest that in the FVTCT‐BALB cell line the synthesis of FV is blocked in some late stage of virus assembly.