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Clonal transformation of human leukocytes by Epstein‐barr virus in soft agar
Author(s) -
Yamamoto Naoki,
Hinuma Yorio
Publication year - 1976
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910170207
Subject(s) - virus , biology , virology , serial dilution , epstein–barr virus , transformation (genetics) , microbiology and biotechnology , medicine , pathology , gene , biochemistry , alternative medicine
The B95‐8 strain of Epstein‐Barr virus (EBV) induced colony formation of human umbilical cord‐blood leukocytes in soft agar medium. One‐hit response relationship between the number of colonies and the virus dose was observed with high dilutions of the virus preparation. However, there was a presumed cell‐killing effect with low dilutions of virus. The colonies were similarly induced, but with a lower efficiency, in adult peripheral blood leukocyte cultures infected with the virus. The colony‐forming activity of EBV was neutralized by anti‐EBV‐positive but not by negative human sera. The cells in colonies were capable of growing continuously and carried EBV‐associated nuclear antigen. Thus, it was evident that the colony formation was caused by clonal transformation by EBV.